Product Name:

Human Granulysin (GNLY) ELISA Kit

Cat.#:

KB12185

Regulatory Status:

RUO

Alternative:

NKG5; LAG-2; TLA519; T-Lymphocyte Activation Gene 519

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.23-15ng/mL

Sensitivity:

0.09ng/mL

Specificity:

This kit recognizes Human GNLY in samples. No significant cross-reactivity or interference between Human GNLY and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| GNLY

Granulysin (GNLY) is a protein expressed in most mammals which functions as an antimicrobial peptide released by killer lymphocytes in cytotoxic granules.It is a pore-forming peptide, as it can puncture a microbial cell wall, allowing for other death-inducing enzymes to enter the microbe and cause microptosis.GNLY is inhibited by cholesterol, and is most effective in helping to kill cholesterol-deficient microbes.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human GNLY spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human GNLY and diluted with Universal Diluent to produce samples with values within the range of the assay.