Product Name:

Human S100 Calcium Binding Protein P (S100P) ELISA Kit

Cat.#:

JL14389

Regulatory Status:

RUO

Alternative:

S100-P; MIG9; S100-E; Migration-inducing gene 9 protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.35ng/mL

Specificity:

This kit recognizes Human S100P in samples. No significant cross-reactivity or interference between Human S100P and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

| S100P

S100 calcium-binding protein P (S100P) is a protein that is encoded by the S100P gene.The protein encoded by this gene is a member of the S100 family of Proteins containing 2 EF-hand calcium-binding motifs. S100 Proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human S100P spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human S100P and diluted with Universal Diluent to produce samples with values within the range of the assay.