Human Trefoil Factor 1 (TFF1) ELISA Kit KB-E1044H

Alternative:

BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer,Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.15ng/mL

Specificity:

This kit recognizes Human TFF1 in samples. No significant cross-reactivity or interference between Human TFF1 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| TFF1

Trefoil factor 1 is a protein that is encoded by the TFF1 gene (also called pS2 gene).Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory Proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TFF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human TFF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.