Product Name:

Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

Cat.#:

KB15107

Regulatory Status:

RUO

Alternative:

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

12.31pg/mL

Specificity:

This kit recognizes Human MFGE8 in samples. No significant cross-reactivity or interference between Human MFGE8 and analogues was observed.

Sample Type:

serum, plasma, cell lysates and other biological fluids

Research Area:

Cancer

| MFGE8

Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a protein that is encoded by the MFGE8 gene.MFGE8 may function as a cell adhesion protein to connect smooth muscle to elastic fibers in arteries. An amyloid fragment of MFGE8 known as medin accumulates in the aorta with aging.[8] MFGE8 in the vasculature of adults can induce recovery from ischemia by facilitating angiogenesis. It has been suggested that antagonizing MFGE8-induced angiogenesis could be a way of fighting cancer.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human MFGE8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human MFGE8 and diluted with Universal Diluent to produce samples with values within the range of the assay.