Product Name:

Human Galectin 7 (Galectin-7) ELISA Kit

Cat.#:

KB14505

Regulatory Status:

RUO

Alternative:

LGALS7; PIG1; TP53I1; HKL-14; Lectin Galactoside-Binding Soluble 7; p53-induced gene 1 protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.04ng/mL

Specificity:

This kit recognizes Human Galectin-7 in samples. No significant cross-reactivity or interference between Human Galectin-7 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| Galectin-7

Galectin-7 is a protein that is encoded by the LGALS7 gene.The galectins are a family of beta-galactoside-binding Proteins implicated in modulating cell–cell and cell–matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). It is moderately repressed by retinoic acid. The protein was found mainly in stratified squamous epithelium. The antigen localized to basal keratinocytes, although it was also found, albeit at lower levels, in the suprabasal layers where it concentrated to areas of cell-to-cell contact.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Galectin-7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human Galectin-7 and diluted with Universal Diluent to produce samples with values within the range of the assay.