Human Mucin 17 (MUC17) ELISA Kit

Alternative:MUC3; Mucin Cell Surface Associated; Small intestinal mucin-3
Detection Range:15.62-1000pg/mL
Sensitivity:6.07pg/mL
Assay Type:Sandwich

Product Name:

Human Mucin 17 (MUC17) ELISA Kit

Cat.#:

KB20042

Regulatory Status:

RUO

Alternative:

MUC3; Mucin Cell Surface Associated; Small intestinal mucin-3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

6.07pg/mL

Specificity:

This kit recognizes Human MUC17 in samples. No significant cross-reactivity or interference between Human MUC17 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Research Area:

Cancer


| MUC17

Mucin-17 is a protein that is encoded by the MUC17 gene.Membrane mucins, such as MUC17, function in epithelial cells to provide cytoprotection, maintain luminal structure, provide signal transduction, and confer antiadhesive properties upon cancer cells that lose their apical/basal polarization.


| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

Concentration (pg/mL)100050025012562.531.2515.620
OD2.281.911.430.970.620.380.220.07
Corrected OD2.211.841.360.90.550.310.15-

 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human MUC17 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10196
Plasma(n=8)92-105101
Tissue Homogenates(n=8)96-108105






 

| Linearity

Samples were spiked with high concentrations of Human MUC17 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 



Serum(n=4)Plasma(n=4)Tissue Homogenates(n=4)
1 : 2Range (%)84-9588-9690-110
Average (%)919396
1 : 4Range (%)89-10387-108105-115
Average (%)9498108







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