Product Name:

Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit

Cat.#:

KB20043

Regulatory Status:

RUO

Alternative:

TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

28.39pg/mL

Specificity:

This kit recognizes Human MUC5AC in samples. No significant cross-reactivity or interference between Human MUC5AC and analogues was observed.

Sample Type:

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| MUC5AC

Mucin-5AC (MUC-5AC) is a protein that is encoded by the MUC5AC gene.MUC-5AC is a large gel-forming glycoprotein. In the respiratory tract, it protects against infection by binding to inhaled pathogens, which are subsequently removed by mucociliary clearance. Overproduction of MUC-5AC can contribute to diseases such as asthma and chronic obstructive pulmonary disease, and has also been associated with greater protection against influenza infection.

 Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human MUC5AC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human MUC5AC and diluted with Universal Diluent to produce samples with values within the range of the assay.