Mouse Growth Regulated Oncogene Beta (GROb) ELISA Kit

Alternative:

CXCL2;SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

6.07pg/mL

Specificity:

This kit recognizes Mouse GROb in samples. No significant cross-reactivity or interference between Mouse GROb and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| GROb

Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells.The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines.CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse GROb spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse GROb and diluted with Universal Diluent to produce samples with values within the range of the assay.