Cat.#:KB-E2086R
Regulatory Status:RUO
Alternative:PLAU; ATF; URK; UK; UP-A; Abbokinase; Urokinase-Type Plasminogen Activator;Plasminogen Activator, Urokinase
Urokinase, also known as urokinase-type plasminogen activator (uPA), is a serine protease present in humans and other animals. The human urokinase protein was discovered, but not named, by McFarlane and Pilling in 1947.[5] Urokinase was originally isolated from human urine, and it is also present in the blood and in the extracellular matrix of many tissues. The primary physiological substrate of this enzyme is plasminogen, which is an inactive form (zymogen) of the serine protease plasmin. Activation of plasmin triggers a proteolytic cascade that, depending on the physiological environment, participates in thrombolysis or extracellular matrix degradation. This cascade had been involved in vascular diseases and cancer progression.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
The recovery of Rat uPA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Samples were spiked with high concentrations of Rat uPA and diluted with Universal Diluent to produce samples with values within the range of the assay.
CATALOGUE