Human Apolipoprotein L Domain Containing 1 (APOLD1) ELISA Kit KB-E0513H

Regulatory Status:RUO

Alternative:VERGE; Vascular early response gene protein;Apolipoprotein L Domain Containing Protein 1

Regulatory Status:

RUO

Alternative:

VERGE; Vascular early response gene protein;Apolipoprotein L Domain Containing Protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.158ng/mL

Specificity:

This kit recognizes Human APOLD1 in samples. No significant cross-reactivity or interference between Human APOLD1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction


| APOLD1


Apolipoprotein L domain containing 1 is a protein that is encoded by the APOLD1 gene. It is located on Chromosome 12.APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)201052.51.250.6250.3120
OD2.331.761.160.70.460.330.240.1
Corrected OD2.231.661.060.60.360.230.14-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human APOLD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)85-10196
Cell Culture Supernates(n=8)92-105103






 

| Linearity


Samples were spiked with high concentrations of Human APOLD1 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)Cell Culture Supernates(n=4)
1 : 2Range (%)84-9588-96
Average (%)9194
1 : 4Range (%)89-10388-108
Average (%)9498








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