Mouse Retinol Binding Protein 4, Plasma (RBP-4) ELISA Kit KB-E1840M

Regulatory Status:

RUO

Alternative:

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.29ng/mL

Specificity:

This kit recognizes Mouse RBP-4 in samples. No significant cross-reactivity or interference between Mouse RBP-4 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

| RBP-4

Retinol binding protein 4, also known as RBP4, is a transporter protein for retinol (vitamin A alcohol). RBP4 has a molecular weight of approximately 21 kDa and is encoded by the RBP4 gene in humans. It is mainly, though not exclusively, synthesized in the liver and circulates in the bloodstream as a hepatokine bound to retinol in a complex with transthyretin. RBP4 has been a drug target for ophthalmology research due to its role in vision. RBP4 may also be involved in metabolic diseases as suggested by recent studies.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse RBP-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse RBP-4 and diluted with Universal Diluent to produce samples with values within the range of the assay.