Mouse Glucoprotein 130 (gp130) ELISA Kit KB-E11058M

Regulatory Status:

RUO

Alternative:

CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6RB; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

12.5-800pg/mL

Sensitivity:

6.19pg/mL

Specificity:

This kit recognizes Mouse gp130 in samples. No significant cross-reactivity or interference between Mouse gp130 and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids

Research Area:

Signal Transduction

| gp130

Glycoprotein 130 (also known as gp130, IL6ST, IL6R-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of the type I cytokine receptor within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse gp130 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse gp130 and diluted with Universal Diluent to produce samples with values within the range of the assay.