Human Synaptic vesicle membrane protein VAT-1 homolog (VAT1) ELISA Kit KB-E3035H

Regulatory Status:

RUO

Alternative:

Vesicle Amine Transport Protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.155ng/mL

Specificity:

This kit recognizes Human VAT1 in samples. No significant cross-reactivity or interference between Human VAT1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| VAT1

Synaptic vesicle membrane protein VAT-1 homolog is a protein that is encoded by the VAT1 gene.Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase Proteins.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human VAT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human VAT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.