Mouse Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5;CD40) ELISA Kit KB-E1430M

Regulatory Status:

RUO

Alternative:

TNFRSF5; CDW40; Bp50; P50; B-cell surface antigen CD40; CD40L receptor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.07ng/mL

Specificity:

This kit recognizes Mouse TNFRSF5;CD40 in samples. No significant cross-reactivity or interference between Mouse TNFRSF5;CD40 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates and other biological fluids

Research Area:

Signal Transduction

| TNFRSF5;CD40

Cluster of differentiation 40, CD40 is a type I transmembrane protein found on antigen-presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.Activated CD4+ T cells primarily exhibit its ligand CD40L/CD154 to antigen-presenting cells including dendritic cells (DCs), B cells, macrophages, classical and non-classical monocytes, on a variety of non-immune cells including platelets and endothelial cells, and on several types of tumor cells.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse TNFRSF5;CD40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse TNFRSF5;CD40 and diluted with Universal Diluent to produce samples with values within the range of the assay.