Human Cold Shock Domain Containing Protein E1 (CSDE1;NRAS) ELISA Kit KB-E2042H

Regulatory Status:

RUO

Alternative:

UNR; NRU; Upstream of NRAS; N-ras upstream gene protein；Cold Shock Domain Containing Protein E1, RNA Binding

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.154ng/mL

Specificity:

This kit recognizes Human CSDE1;NRAS in samples. No significant cross-reactivity or interference between Human CSDE1;NRAS and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| CSDE1;NRAS

Cold shock domain-containing protein E1 is a protein that is encoded by the CSDE1 gene.Enables RISC complex binding activity; RNA stem-loop binding activity; and lncRNA binding activity. Involved in IRES-dependent viral translational initiation; regulation of gene expression; and stress granule assembly. Located in Golgi apparatus; cytosol; and plasma membrane. Part of CRD-mediated mRNA stability complex and mCRD-mediated mRNA stability complex.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CSDE1;NRAS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human CSDE1;NRAS and diluted with Universal Diluent to produce samples with values within the range of the assay.