Mouse Lactoferrin (LTF) ELISA Kit KB-E14136M

Regulatory Status:RUO
Alternative:HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

Alternative:

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

30.35pg/mL

Specificity:

This kit recognizes Mouse LTF in samples. No significant cross-reactivity or interference between Mouse LTF and analogues was observed.

Sample Type:

Serum, plasma, breast milk and other biological fluids

Research Area:

Cancer


| LTF


Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin is also present in secondary granules of PMNs and is secreted by some acinar cells. Lactoferrin can be purified from milk or produced recombinantly.Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses.It is constantly produced and released into saliva, tears, as well as seminal and vaginal fluid.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


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| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Mouse LTF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


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| Linearity


Samples were spiked with high concentrations of Mouse LTF and diluted with Universal Diluent to produce samples with values within the range of the assay.


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