Product Name:

Mouse N-Acetyl Beta-D-Glucosaminidase (NAG) ELISA Kit

Cat.#:

KB11476

Regulatory Status:

RUO

Alternative:

N-Acetyl Beta-D-Glucosaminidase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.79ng/mL

Specificity:

This kit recognizes Mouse NAG in samples. No significant cross-reactivity or interference between Mouse NAG and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

| NAG

N-Acetyl Beta-D-Glucosaminidase (NAG) is a mesophilic hydrolase that specifically hydrolyzes N-acetyl-glucosides. One of the primary functions of the enzyme is to target and hydrolyze oligosaccharides containing chitin. In this chitinase function, the enzyme contributes to the ability of many organisms to break down chitin-containing molecules and subsequently digest or re-uptake environmental chitin, carbon, or nitrogen. The enzyme's crystal structure varies slightly across organisms, but is characterized by three or four domains with one active site.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse NAG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse NAG and diluted with Universal Diluent to produce samples with values within the range of the assay.